Open AccessControlling Proteome Degradation in Daphnia pulex
Author(s) -
Kemp Catherine Jarrett,
Kültz Dietmar
Publication year - 2012
Publication title -
journal of experimental zoology part a: ecological genetics and physiology
Language(s) - English
Resource type - Journals
eISSN - 1932-5231
pISSN - 1932-5223
DOI - 10.1002/jez.1766
Subject(s) - daphnia pulex , biology , proteome , proteolysis , salinity , pulex , daphnia , homogenization (climate) , sodium , biochemistry , enzyme , zoology , ecology , chemistry , crustacean , biodiversity , organic chemistry
Abstract An attempted proteomic study of Daphnia pulex response to sodium chloride exposure revealed an unexpected result: very strong proteome degradation previously observed in D. pulex is halted when animals have been exposed to elevated salinity prior to protein extraction. Further characterization of conditions controlling proteome degradation shows that the proteolytic factor(s) is/are present in an active form under chemically denaturing conditions in extract from control “normal/healthy” animals, and can be negatively regulated by prior in vivo salinity exposure of animals or inhibited by post‐homogenization heating of samples. We interpret these data as evidence for salinity‐induced inhibition of proteolysis in Daphnia . J. Exp. Zool. 317A:645–651, 2012. © 2012 Wiley Periodicals, Inc.