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Human iPSC‐Vascular smooth muscle cell spheroids demonstrate size‐dependent alterations in cellular viability and secretory function
Author(s) -
Islam Sara,
Parker Jackson,
Dash Biraja C.,
Hsia Henry C.
Publication year - 2022
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.37423
Subject(s) - paracrine signalling , spheroid , microbiology and biotechnology , induced pluripotent stem cell , secretion , vascular smooth muscle , autocrine signalling , regenerative medicine , wound healing , cell , dermal fibroblast , biology , cell type , fibroblast , stem cell , cell culture , immunology , smooth muscle , endocrinology , receptor , biochemistry , embryonic stem cell , gene , genetics
Human‐induced pluripotent stem cells (hiPSC) and their differentiated vascular cells have been revolutionizing the field of regenerative wound healing. These cells are shown to be rejuvenated with immense potentials in secreting paracrine factors. Recently, hiPSC‐derived vascular smooth muscle cells (hiPSC‐VSMC) have shown regenerative wound healing ability via their paracrine secretion. The quest to modulate the secretory function of these hiPSC‐VSMC is an ongoing effort and involves the use of both biochemical and biophysical stimuli. This study explores the development and optimization of a reproducible, inexpensive protocol to form hiPSC‐VSMC derived spheroids to investigate the implications of spheroid size on viability and paracrine secretion. Our data show the successful formation of different sizes of spheroids using various amount of hiPSC‐VSMC. The hiPSC‐VSMC spheroids formed with 10,000 cells strike an ideal balance between overall cell health and maximal paracrine secretion. The conditioned medium from these spheroids was found to be bioactive in enhancing human dermal fibroblast cell proliferation and migration. This research will inform future studies on the optimal spheroid size for regenerative wound healing applications.