Open AccessMucin secretion in inflammatory bowel disease: Comparison of a macrophage‐derived mucin secretagogue (MMS‐68) to conventional secretagogues
Author(s) -
Sperber Kirk,
Shim Jan,
Mehra Munisha,
Lin Annica,
George Italas,
Ogata Shunichiro,
Mayer Lloyd,
Itzkowitz Steven
Publication year - 1998
Publication title -
inflammatory bowel diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.932
H-Index - 146
eISSN - 1536-4844
pISSN - 1078-0998
DOI - 10.1002/ibd.3780040104
Subject(s) - mucin , secretagogue , intestinal mucosa , secretion , inflammatory bowel disease , goblet cell , colitis , mucus , chemistry , medicine , pathology , biology , epithelium , ecology , disease
We have described a novel macrophage‐derived mucin secretagogue (MMS‐68) that mediates mucin secretion in colon cancer cell lines and explants of normal and inflammatory bowel disease (IBD) mucosa. we compared MMS‐68 induced mucin release with other known intestinal mucin secretagogues in normal colon explants and in the HT‐29 colon cancer cell line, and to study the effects of MMS‐68 on mucin release from inflamed and uninflamed ulcerative colitis (UC) and Crohn's disease (CD) mucosa. In normal colonic explants and HT‐29 cells, each of the secretagogues including MMS‐68‐induced mucin release two‐ to fivefold more than culture medium alone. In HT‐29 cells, MMS‐68 plus leukotriene C 4 (LTC 4 ) induced a 50% increase in mucin release over either secretagogue alone, and MMS‐68 plus platelet‐activating factor (PAF) markedly enhanced mucin release by eightfold over either secretagogue. In colonic explants from patients with UC and CD, the mucin release in response to MMS‐68 was similar to that of normal colonic explants. Likewise, in isolated epithelial cells from CD and UC (whether involved or uninvolved), MMS‐68‐induced release was similar to that of epithelial cells isolated from normal colonic mucosa. The number of MMS‐68‐producing macrophages was lower in uninflamed UC mucosa compared with inflamed UC mucosa and CD mucosa. The mucin secretagogue activity of MMS‐68 is comparable to that of other known secretagogues, and PAF can have a synergistic effect on this activity. Whole tissue explants and isolated colonic epithelial cells from patients with IBD respond at least as well as their normal counterparts to MMS‐68. MMS‐68 may play a role in mucin secretion in normal and inflamed colonic tissue.