Open AccessThe role of reactive oxygen metabolites in ulcerative colitis
Author(s) -
Dagli Ülkü,
Balk Mevhibe,
Yücel Doǧan,
Ülker Aysel,
Över Hülya,
Saydam Gülsevim,
Çahin Burhan
Publication year - 1997
Publication title -
inflammatory bowel diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.932
H-Index - 146
eISSN - 1536-4844
pISSN - 1078-0998
DOI - 10.1002/ibd.3780030404
Subject(s) - ulcerative colitis , myeloperoxidase , malondialdehyde , superoxide dismutase , thiobarbituric acid , inflammatory bowel disease , colitis , reactive oxygen species , gastroenterology , medicine , chemistry , pathology , inflammation , biochemistry , oxidative stress , disease , lipid peroxidation
Reactive oxygen metabolites (ROMs) contribute to tissue injury in inflammatory bowel disease. The aim of this study is to examine the role of ROMs in the tissue injury in ulcerative colitis (UC). The study group consisted of 27 patients with UC (14 active, 13 quiescent) and a control group of 10 patients with various anal diseases. We measured the content of malondialdehyde (MDA), superoxide dismutase (SOD), and myeloperoxidase (MPO) in colorectal biopsies. MDA was measured by the thiobarbituric acid assay. SOD and MPO were measured using the nitro blue tetrazolium and o ‐dianisidine methods, respectively. The MDA, SOD, and MPO tissue levels were significantly different between the patients with active UC, the patients with quiescent UC, and the control subjects (p < 0.001). A positive correlation was found between the tissue concentrations of MDA and MPO and the activity of the disease (p < 0.001). The SOD tissue concentrations were negatively correlated with the disease activity ( r = −0.507, p < 0.05).