Open AccessEffect of inflammatory bowel disease classification changes on NOD2 genotype–phenotype associations in a population‐based cohort
Author(s) -
Gearry Richard B.,
Roberts Rebecca L.,
Burt Michael J.,
Frampton Chris M.A.,
Chapman Bruce A.,
Collett Judith A.,
Shirley Philippa,
Allington Melanie D.E.,
Kennedy Martin A.,
Barclay Murray L.
Publication year - 2007
Publication title -
inflammatory bowel diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.932
H-Index - 146
eISSN - 1536-4844
pISSN - 1078-0998
DOI - 10.1002/ibd.20190
Subject(s) - nod2 , inflammatory bowel disease , ulcerative colitis , medicine , genotype , odds ratio , cohort , genotyping , crohn's disease , population , gastroenterology , allele , disease , genetics , biology , environmental health , gene
Abstract Background: NOD2 mutations are associated with Crohn's disease (CD) in Caucasian clinic‐based cohorts. Data from population‐based cohorts are limited. Clinic‐based studies may overestimate this association. Genotype–phenotype relationships are yet to be assessed using the Montreal classification. We hypothesized that the NOD2 –CD association would be weaker in a population‐based cohort, and that the Montreal classification would strengthen genotype–phenotype associations. Methods: A population‐based case‐control study was performed including 91% of all people in Canterbury, New Zealand, with inflammatory bowel disease (IBD); NOD2 genotyping was performed and patients were phenotyped according to the Vienna and Montreal classification systems. Results: The NOD2 genotype was available on 684 CD, 643 ulcerative colitis (UC), 36 indeterminate colitis/IBDU (IBD unclassified) patients, and 201 controls. Control frequencies for the 702W, 908R, and 1007fs alleles were 0.030, 0.012, and 0.010, respectively, compared with 0.074, 0.027, and 0.040 for CD. The 702W ( P = 0.001) and 1007fs ( P = 0.002) alleles were significantly associated with CD. Younger age of diagnosis (<17 years) was associated with 1 (odds ratio (OR) 1.9 [95% confidence intervals 0.98–3.6]) or 2 (OR 6.5 [2.3–18.6]) NOD2 mutations compared with diagnosis >40 years. Ileal disease was most strongly associated with NOD2 mutations (1 mutation OR 3.9 [2.4–6.3], 2 mutations OR 6.7 [2.4–18.5]). Penetrating disease was associated with NOD2 mutations using the Montreal but not the Vienna classification. Conclusions: The association between NOD2 mutations and CD was found to be weaker in our population‐based cohort than in previous studies that used referral‐based cohorts. Application of the Montreal classification led to a strengthening of the NOD2 genotype–phenotype association. (Inflamm Bowel Dis 2007)