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Comparative assessment of electronic nicotine delivery systems aerosol and cigarette smoke on endothelial cell migration: The Replica Project
Author(s) -
Caruso Massimo,
Emma Rosalia,
Distefano Alfio,
Rust Sonja,
Poulas Konstantinos,
Giordano Antonio,
Volarevic Vladislav,
Mesiakaris Konstantinos,
Boffo Silvia,
Arsenijevic Aleksandar,
Karanasios Georgios,
Pulvirenti Roberta,
Ilic Aleksandar,
Canciello Angelo,
Zuccarello Pietro,
Ferrante Margherita,
Polosa Riccardo,
Li Volti Giovanni
Publication year - 2023
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.3349
Subject(s) - aerosol , cigarette smoke , endothelial stem cell , umbilical vein , nicotine , smoke , electronic cigarette , endothelium , chemistry , wound healing , medicine , pharmacology , in vitro , toxicology , biology , immunology , biochemistry , organic chemistry
Cigarette smoking is associated with impairment of repair mechanisms necessary for vascular endothelium homeostasis. Reducing the exposure to smoke toxicants may result in the mitigation of the harmful effect on the endothelium and cardiovascular disease development. Previous investigations evaluated in vitro the effect of electronic cigarette (EC) compared with cigarette smoke demonstrating a significant reduction in human umbilical vein endothelial cells (HUVECs) migration inhibition following EC aerosol exposure. In the present study, we replicated one of these studies, evaluating the effects of cigarette smoke on endothelial cell migration compared with aerosol from EC and heated tobacco products (HTPs). We performed an in vitro scratch wound assay on endothelial cells with a multi‐center approach (ring‐study) to verify the robustness and reliability of the results obtained in the replicated study, also testing the effect of aerosol from two HTPs on endothelial cells. Consistently with the original study, we observed a substantial reduction of the effects of aerosol from EC and HTPs on endothelial cell migration compared with cigarette smoke. While cigarette smoke reduced endothelial wound healing ability already at low concentrations (12.5%) and in a concentration‐dependent manner, EC and HTPs aerosol showed no effect on endothelial cells until 80%–100% concentrations. In conclusion, our study further confirms the importance of EC and tobacco heated products as a possible harm reduction strategy for cardiovascular diseases development in smokers.