White blood cell differentiation using a solid state flow cytometer | Zendy

Doornbos R. M. P. | Zendy; Hennink E. J. | Zendy; Putman C. A. J. | Zendy; De Grooth B. G. | Zendy; Greve J. | Zendy

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White blood cell differentiation using a solid state flow cytometer

Author(s) -

Doornbos R. M. P.,

Hennink E. J.,

Putman C. A. J.,

De Grooth B. G.,

Greve J.

Publication year - 1993

Publication title -

cytometry

Language(s) - English

Resource type - Journals

eISSN - 1097-0320

pISSN - 0196-4763

DOI - 10.1002/cyto.990140602

Subject(s) - detector , laser , photomultiplier , avalanche photodiode , optics , photodiode , diode , materials science , flow cytometry , optoelectronics , helium , physics , biology , microbiology and biotechnology , atomic physics

A flow cytometer using a solid state light source and detector was designed and built. For illumination of the sample stream two types of diode lasers (670 nm and 780 nm) were tested in a set‐up designed to differentiate human leukocytes by means of light scattering. The detector is an avalanche photodiode, which was used to detect the weak scattered light in the orthogonal direction. The new flow cytometer set‐up is very small, relatively cheap and yields similar results as a standard flow cytometer set‐up using a helium‐neon laser and photomultipliers. © 1993 Wiley‐Liss, Inc.

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