Open AccessGeneration and Fusion of Human Cortical and Medial Ganglionic Eminence Brain Organoids
Author(s) -
Xiang Yangfei,
Yoshiaki Tanaka,
Patterson Benjamin,
Cakir Bilal,
Kim KunYong,
Cho Yee Sook,
Park InHyun
Publication year - 2018
Publication title -
current protocols in stem cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 28
eISSN - 1938-8969
pISSN - 1941-7322
DOI - 10.1002/cpsc.61
Subject(s) - organoid , ganglionic eminence , biology , neuroscience , human brain , cortex (anatomy) , embryonic stem cell , anatomy , central nervous system , cerebrum , genetics , gene
Three‐dimensional brain organoid culture has become an essential tool for investigating human brain development and modeling neurological disorders during the past few years. Given specific regionalization during brain development, it is important to produce distinct brain organoids that represent different brain regions and their interactions. The authors recently established a platform to generate brain organoids resembling the medial ganglionic eminence (MGE), a specific brain region responsible for interneurogenesis, and found that when these organoids were fused with organoids resembling the cortex, the resulting organoids enabled modeling of interneuron migration in the brain. This unit describes four basic protocols that have been successfully applied by the authors, covering the generation of embryonic bodies with neuroectodermal fate, the production of human MGE organoids and cortical organoids, and the fusion of these two organoids. © 2018 by John Wiley & Sons, Inc.