Open AccessComprehensive Protocols for CRISPR/Cas9‐based Gene Editing in Human Pluripotent Stem Cells
Author(s) -
Santos David P.,
Kiskinis Evangelos,
Eggan Kevin,
Merkle Florian T.
Publication year - 2016
Publication title -
current protocols in stem cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 28
eISSN - 1938-8969
pISSN - 1941-7322
DOI - 10.1002/cpsc.15
Subject(s) - crispr , genome editing , induced pluripotent stem cell , cas9 , biology , computational biology , gene , human induced pluripotent stem cells , genetics , embryonic stem cell
Genome editing of human pluripotent stem cells (hPSCs) with the CRISPR/Cas9 system has the potential to revolutionize hPSC‐based disease modeling, drug screening, and transplantation therapy. Here, we aim to provide a single resource to enable groups, even those with limited experience with hPSC culture or the CRISPR/Cas9 system, to successfully perform genome editing. The methods are presented in detail and are supported by a theoretical framework to allow for the incorporation of inevitable improvements in the rapidly evolving gene‐editing field. We describe protocols to generate hPSC lines with gene‐specific knock‐outs, small targeted mutations, or knock‐in reporters. © 2016 by John Wiley & Sons, Inc.