Open AccessHybridization Histochemistry of Neural Transcripts
Author(s) -
Young W. Scott,
Song June,
Mezey Éva
Publication year - 2018
Publication title -
current protocols in neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 40
eISSN - 1934-8576
pISSN - 1934-8584
DOI - 10.1002/cpns.39
Subject(s) - rna , in situ hybridization , microbiology and biotechnology , computational biology , biology , messenger rna , biochemistry , chemistry , gene
This unit presents protocols to locate RNA transcripts in tissues. Numerous approaches are detailed, including those that use radiolabeled or colorimetric probes. Also, the probes may be modified oligodeoxynucleotides, singly or in pairs, as well as ribonucleic acids. High sensitivity and specificity are obtained, especially with sets of oligodeoxynucleotide pairs. © 2018 by John Wiley & Sons, Inc.