Open AccessMultisite Electrophysiology Recordings in Mice to Study Cross‐Regional Communication During Anxiety
Author(s) -
Harris Alexander Z.,
Golder Danielle,
Likhtik Ekaterina
Publication year - 2017
Publication title -
current protocols in neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 40
eISSN - 1934-8576
pISSN - 1934-8584
DOI - 10.1002/cpns.32
Subject(s) - optogenetics , troubleshooting , neuroscience , electrophysiology , local field potential , anxiety , computer science , psychology , psychiatry , operating system
Recording neural activity in awake, freely moving mice is a powerful and flexible technique for dissecting the neural circuit mechanisms underlying pathological behavior. This unit describes protocols for designing a drive and recording single neurons and local field potentials during anxiety‐related paradigms. We also include protocols for integrating pharmacologic and optogenetic means for circuit manipulations, which, when combined with electrophysiological recordings, demonstrate input‐specific and cell‐specific contributions to circuit‐wide activity. We discuss the planning, execution, and troubleshooting of physiology experiments during anxiety‐like behavior. © 2017 by John Wiley & Sons, Inc.