Open AccessPeripheral Neuropathy in Mouse Models of Diabetes
Author(s) -
Jolivalt Corinne G.,
Frizzi Katie E.,
Guernsey Lucie,
Marquez Alex,
Ochoa Joseline,
Rodriguez Maria,
Calcutt Nigel A.
Publication year - 2016
Publication title -
current protocols in mouse biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.77
H-Index - 15
ISSN - 2161-2617
DOI - 10.1002/cpmo.11
Subject(s) - diabetes mellitus , peripheral neuropathy , medicine , neuropathic pain , polyneuropathy , type 2 diabetes , diabetic neuropathy , complication , peripheral , neuroscience , bioinformatics , intensive care medicine , endocrinology , anesthesia , psychology , biology
Abstract Peripheral neuropathy is a frequent complication of chronic diabetes that most commonly presents as a distal degenerative polyneuropathy with sensory loss. Around 20% to 30% of such patients may also experience neuropathic pain. The underlying pathogenic mechanisms are uncertain, and therapeutic options are limited. Rodent models of diabetes have been used for more than 40 years to study neuropathy and evaluate potential therapies. For much of this period, streptozotocin‐diabetic rats were the model of choice. The emergence of new technologies that allow relatively cheap and routine manipulations of the mouse genome has prompted increased use of mouse models of diabetes to study neuropathy. In this article, we describe the commonly used mouse models of type 1 and type 2 diabetes, and provide protocols to phenotype the structural, functional, and behavioral indices of peripheral neuropathy, with a particular emphasis on assays pertinent to the human condition. © 2016 by John Wiley & Sons, Inc.