Mucor circinelloides : Growth, Maintenance, and Genetic Manipulation | Zendy

Vellanki Sandeep | Zendy; NavarroMendoza Maria Isabel | Zendy; Garcia Alexis | Zendy; Murcia Laura | Zendy; PerezArques Carlos | Zendy; Garre Victoriano | Zendy; Nicolas Francisco E. | Zendy; Lee Soo Chan | Zendy

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Mucor circinelloides : Growth, Maintenance, and Genetic Manipulation

Author(s) -

Vellanki Sandeep,

NavarroMendoza Maria Isabel,

Garcia Alexis,

Murcia Laura,

PerezArques Carlos,

Garre Victoriano,

Nicolas Francisco E.,

Lee Soo Chan

Publication year - 2018

Publication title -

current protocols in microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.813

H-Index - 35

eISSN - 1934-8533

pISSN - 1934-8525

DOI - 10.1002/cpmc.53

Subject(s) - mucor circinelloides , biology , fungus , mucorales , mucormycosis , mucor , gene silencing , filamentous fungus , rna interference , model organism , genetics , gene , botany , rna , aspergillus , medicine , pathology

Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a model organism for studying several biological processes, such as light responses and RNA interference–mediated gene silencing. Over the past decade, the increasing number of molecular tools has also allowed us to manipulate the genome of this fungus. This article outlines the fundamental protocols for the in vitro growth, maintenance, and genetic manipulation of M. circinelloides in the laboratory. © 2018 by John Wiley & Sons, Inc.

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