Open AccessDetection and Genotyping of Human Papillomaviruses from Archival Formalin‐Fixed Tissue Samples
Author(s) -
Doorslaer Koenraad,
Chen Zigui,
McBride Alison A.
Publication year - 2016
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/cpmc.16
Subject(s) - genotyping , biology , dna extraction , polymerase chain reaction , amplicon , human papillomavirus , dna sequencing , dna , virology , genotype , genetics , gene , medicine
Pathology departments routinely process and store formalin‐fixed, paraffin‐embedded (FFPE) tissue samples for clinical diagnosis. These collections often contain decades’ worth of samples and represent a treasure trove of specimens that can be analyzed for retrospective epidemiological studies, diagnostics, and pathogen discovery. Accurate amplification and sequencing of DNA from these samples is critical for the usability of these FFPE samples. Here we present a collection of protocols that describe extraction of DNA from FFPE tissues, PCR amplification of human papillomavirus DNA, and subsequent genotyping of the infecting virus. © 2016 by John Wiley & Sons, Inc.