Differential Radial Capillary Action of Ligand Assay (DRaCALA) | Zendy

Seminara Anna B. | Zendy; Turdiev Asan | Zendy; Turdiev Husan | Zendy; Lee Vincent T. | Zendy

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Differential Radial Capillary Action of Ligand Assay (DRaCALA)

Author(s) -

Seminara Anna B.,

Turdiev Asan,

Turdiev Husan,

Lee Vincent T.

Publication year - 2019

Publication title -

current protocols in molecular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.533

H-Index - 42

eISSN - 1934-3647

pISSN - 1934-3639

DOI - 10.1002/cpmb.84

Subject(s) - nucleic acid , ligand (biochemistry) , chemistry , computational biology , small molecule , macromolecule , ligand binding assay , biochemistry , biophysics , biology , receptor

Protein interactions with nucleic acids are important for the synthesis, regulation, and stability of macromolecules. While a number of assays are available for interrogating these interactions, the differential radial capillary action of ligand assay (DRaCALA) has been developed as an easy and flexible platform that allows for the study of individual interactions when carrying out high‐throughput screening for novel binding proteins and small molecule inhibitors. In this article, we describe the principle of DRaCALA and methods that utilize DRaCALA to determine the affinity and specificity of individual protein‐nucleic acid interactions as well as uses for screening for binding proteins and chemical inhibitors. © 2018 by John Wiley & Sons, Inc.

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