RNAi Screening: Automated High‐Throughput Liquid RNAi Screening in  Caenorhabditis elegans | Zendy

Jagadeesan Sakthimala | Zendy; Hakkim Abdul | Zendy

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RNAi Screening: Automated High‐Throughput Liquid RNAi Screening in Caenorhabditis elegans

Author(s) -

Jagadeesan Sakthimala,

Hakkim Abdul

Publication year - 2018

Publication title -

current protocols in molecular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.533

H-Index - 42

eISSN - 1934-3647

pISSN - 1934-3639

DOI - 10.1002/cpmb.65

Subject(s) - rna interference , caenorhabditis elegans , biology , gene , genetics , gene silencing , genome , computational biology , genetic screen , high throughput screening , rna , phenotype

RNAi is a powerful reverse genetics tool that has revolutionized genetic studies in model organisms. The bacteriovorous nematode Caenorhabditis elegans can be genetically manipulated by feeding it an Escherichia coli strain that expresses a double‐stranded RNA (dsRNA) corresponding to a C. elegans gene, which leads to systemic silencing of the gene. This unit describes protocols for performing an automated high‐throughput RNAi screen utilizing a full‐genome C. elegans RNAi library. The protocols employ liquid‐handling robotics and 96‐well plates. © 2018 by John Wiley & Sons, Inc.

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