Pyroptosis Induction and Detection | Zendy

den Hartigh Andreas B. | Zendy; Fink Susan L. | Zendy

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Pyroptosis Induction and Detection

Author(s) -

den Hartigh Andreas B.,

Fink Susan L.

Publication year - 2018

Publication title -

current protocols in immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.618

H-Index - 43

eISSN - 1934-368X

pISSN - 1934-3671

DOI - 10.1002/cpim.52

Subject(s) - pyroptosis , inflammasome , caspase 1 , inflammation , protease , microbiology and biotechnology , cysteine protease , programmed cell death , chemistry , caspase , apoptosis , biology , immunology , biochemistry , enzyme

Pyroptosis is a form of programmed pro‐inflammatory cell death that plays a protective role in the host response to infection, but can also promote pathogenic inflammation. Pyroptosis is mediated by the cysteine protease, caspase‐1. Caspase‐1 cleaves gasdermin D, releasing the N‐terminal pore‐forming domain, which inserts into the plasma membrane and drives osmotic lysis. Caspase‐1 also proteolytically activates the inflammatory cytokines interleukin 1β (IL‐1β) and IL‐18. This unit describes methods for stimulating pyroptosis and assessing subsequent loss of plasma membrane integrity. We also describe an ELISA to quantify released IL‐1β. These methods can be applied to many different types of experiments. © 2018 by John Wiley & Sons, Inc.

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