Open AccessIntravital Imaging of Neuroimmune Interactions Through a Thinned Skull
Author(s) -
Manglani Monica,
McGavern Dorian B.
Publication year - 2018
Publication title -
current protocols in immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 43
eISSN - 1934-368X
pISSN - 1934-3671
DOI - 10.1002/cpim.46
Subject(s) - meninges , skull , neuroscience , traumatic brain injury , central nervous system , immune system , neuroimaging , biology , regeneration (biology) , pathology , medicine , anatomy , microbiology and biotechnology , immunology , psychiatry
Innate and adaptive immune interactions within the central nervous system (CNS) and surrounding meninges contribute significantly to neural homeostasis as well as a variety of different neurological disorders. Two‐photon laser scanning microscopy is a deep tissue imaging technique that provides a means to image immune cell dynamics and interactions in the living CNS with high spatial and temporal resolution. Optical access to the brain and meninges can be achieved through the creation of thinned skull windows, which can be made without inducing damage and inflammation in the underlying tissue. This protocol provides guidance on how to create a thinned skull window without causing CNS injury. We also describe a highly reproducible method to induce a mild traumatic brain injury using the thinned skull approach. © 2018 by John Wiley & Sons, Inc.