Droplet Digital PCR with EvaGreen Assay: Confirmational Analysis of Structural Variants | Zendy

Tai Angela C. | Zendy; Parfenov Michael | Zendy; Gorham Joshua M. | Zendy

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Droplet Digital PCR with EvaGreen Assay: Confirmational Analysis of Structural Variants

Author(s) -

Tai Angela C.,

Parfenov Michael,

Gorham Joshua M.

Publication year - 2018

Publication title -

current protocols in human genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.282

H-Index - 30

eISSN - 1934-8258

pISSN - 1934-8266

DOI - 10.1002/cphg.58

Subject(s) - digital polymerase chain reaction , computational biology , polymerase chain reaction , microbiology and biotechnology , biology , genetics , chemistry , gene

DNA structural variants can be analyzed by droplet digital PCR (ddPCR), a water‐oil microfluidics and fluorescence technology to quantify target nucleic acids with extreme precision and sensitivity. Traditional ddPCR uses expensive fluorescent oligonucleotide probes that require extensive optimization. Here we describe a variation of ddPCR using a DNA‐binding dye (EvaGreen), whose properties allow target products to be effectively quantified at a significantly lower cost. © 2018 by John Wiley & Sons, Inc.

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