Open AccessPseudo‐Ligandless Click Chemistry for Oligonucleotide Conjugation
Author(s) -
Mack Stephanie,
Fouz Munira F.,
Dey Sourav K.,
Das Subha R.
Publication year - 2016
Publication title -
current protocols in chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 14
ISSN - 2160-4762
DOI - 10.1002/cpch.1
Subject(s) - click chemistry , chemistry , cycloaddition , azide , alkyne , combinatorial chemistry , aqueous solution , oligonucleotide , aqueous medium , organic chemistry , catalysis , dna , biochemistry
Abstract Particularly for its use in bioconjugations, the copper‐catalyzed (or copper‐promoted) azide‐alkyne cycloaddition (CuAAC) reaction or ‘click chemistry’, has become an essential component of the modern chemical biologist's toolbox. Click chemistry has been applied to DNA, and more recently, RNA conjugations, and the protocols presented here can be used for either. The reaction can be carried out in aqueous buffer, and uses acetonitrile as a minor co‐solvent that serves as a ligand to stabilize the copper. The method also includes details on the analysis of the reaction product. © 2016 by John Wiley & Sons, Inc.