Open AccessMyosin‐Va mediates RNA distribution in primary fibroblasts from multiple organs
Author(s) -
Salerno Verônica P.,
Calliari Aldo,
Provance D. William,
SoteloSilveira José R.,
Sotelo José R.,
Mercer John A.
Publication year - 2008
Publication title -
cell motility and the cytoskeleton
Language(s) - English
Resource type - Journals
eISSN - 1097-0169
pISSN - 0886-1544
DOI - 10.1002/cm.20272
Subject(s) - biology , myosin , rna , microbiology and biotechnology , cytoskeleton , actin , messenger rna , rna splicing , alternative splicing , mutant , null cell , cell , genetics , gene
Myosin‐Va has been shown to have multiple functions in a variety of cell types, including a role in RNA transport in neurons. Using primary cultures of cells from organs of young dilute‐lethal (Myo5a d‐l /Myo5a d‐l ) null mutant mice and wild‐type controls, we show that in some, but not all, tissues, RNA distribution is dramatically different in the homozygous null mutant cells. The dependence of RNA localization on myosin‐Va correlates with the relative abundance of the brain‐specific splicing pattern of the myosin‐Va tail. We also show that myosin‐Va is involved in RNA localization soon after synthesis, because the effects of its absence are diminished for RNAs that are more than 30 min old. Finally, we show that localization of β‐actin mRNA is significantly changed by the absence of myosin‐Va. These results suggest that myosin‐Va is involved in a transient transport or tethering function in the perinuclear region. Cell Motil. Cytoskeleton2008. © 2008 Wiley‐Liss, Inc.