Open AccessSynthetic peptide and protein domain arrays prepared by the SPOT technology
Author(s) -
SchneiderMergener Jens
Publication year - 2001
Publication title -
comparative and functional genomics
Language(s) - English
Resource type - Journals
eISSN - 1532-6268
pISSN - 1531-6912
DOI - 10.1002/cfg.99
Subject(s) - hot spot (computer programming) , domain (mathematical analysis) , peptide , computer science , leaf spot , nanotechnology , chemistry , biology , biochemistry , materials science , operating system , mathematics , mathematical analysis , botany
The SPOT ™ technology for highly parallel synthesis of peptides on flat surfaces in array type format has evolved into a versatile toolbox for a variety of applications in proteomics such as mapping protein‐protein interactions and profiling the substrate specificity of enzymes such as kinases and proteases. Originally developed for the synthesis of short overlapping peptide sequences for mapping antibody epitopes this technology has recently been extended to the synthesis of functional protein domains. This opens up a variety of future applications such as target identification and protein expression profiling. Copyright © 2001 John Wiley & Sons, Ltd.