Open AccessThe complete mitochondrial genome of Yarrowia lipolytica
Author(s) -
Kerscher Stefan,
Durstewitz Gregor,
Casaregola Serge,
Gaillardin Claude,
Brandt Ulrich
Publication year - 2001
Publication title -
comparative and functional genomics
Language(s) - English
Resource type - Journals
eISSN - 1532-6268
pISSN - 1531-6912
DOI - 10.1002/cfg.72
Subject(s) - yarrowia , biology , genetics , pseudogene , gene , genome , mitochondrial dna , reading frame , stop codon , open reading frame , intron , peptide sequence
Abstract We here report the complete nucleotide sequence of the 47.9 kb mitochondrial (mt) genome from the obligate aerobic yeast Yarrowia lipolytica . It encodes, all on the same strand, seven subunits of NADH:ubiquinone oxidoreductase (ND1‐6, ND4L), apocytochrome b (COB), three subunits of cytochrome oxidase (COX1, 2, 3), three subunits of ATP synthetase (ATP6, 8 and 9), small and large ribosomal RNAs and an incomplete set of tRNAs. The Y. lipolytica mt genome is very similar to the Hansenula wingei mt genome, as judged from blocks of conserved gene order and from sequence homology. The extra DNA in the Y. lipolytica mt genome consists of 17 group 1 introns and stretches of A+T‐rich sequence, interspersed with potentially transposable GC clusters. The usual mould mt genetic code is used. Interestingly, there is no tRNA able to read CGN (arginine) codons. CGN codons could not be found in exonic open reading frames, whereas they do occur in intronic open reading frames. However, several of the intronic open reading frames have accumulated mutations and must be regarded as pseudogenes. We propose that this may have been triggered by the presence of untranslatable CGN codons. This sequence is available under EMBL Accession No. AJ307410. Copyright © 2001 John Wiley & Sons, Ltd.