Open AccessShotgun cloning of transposon insertions in the genome of Caenorhabditis elegans
Author(s) -
van der Linden Alexander M.,
Plasterk Ronald H. A.
Publication year - 2004
Publication title -
comparative and functional genomics
Language(s) - English
Resource type - Journals
eISSN - 1532-6268
pISSN - 1531-6912
DOI - 10.1002/cfg.392
Subject(s) - caenorhabditis elegans , transposable element , shotgun sequencing , genome , biology , cloning (programming) , genetics , computational biology , shotgun , caenorhabditis , dna transposable elements , computer science , gene , programming language
We present a strategy to identify and map large numbers of transposon insertions in the genome of Caenorhabditis elegans . Our approach makes use of the mutator strain mut‐7 , which has germline‐transposition activity of the Tc1/mariner family of transposons, a display protocol to detect new transposon insertions, and the availability of the genomic sequence of C. elegans . From a pilot insertional mutagenesis screen, we have obtained 351 new Tc1 transposons inserted in or near 219 predicted C. elegans genes. The strategy presented provides an approach to isolate insertions of natural transposable elements in many C. elegans genes and to create a large‐scale collection of C. elegans mutants. Copyright © 2004 John Wiley & Sons, Ltd.