Open AccessThree novel pigmentation mutants generated by genome‐wide random ENU mutagenesis in the mouse
Author(s) -
Tsipouri Vicky,
Curtin John A.,
Nolan Pat M.,
Vizor Lucie,
Parsons Claire A.,
Clapham Colin M.,
Latham Ian D.,
Rooke Lesley J.,
Martin Jo E.,
Peters Jo,
Hunter A. Jackie,
Rogers Derek,
Rastan Sohaila,
Brown Steve D.M.,
Fisher Elizabeth M.C.,
Spurr Nigel K.,
Gray Ian C.
Publication year - 2004
Publication title -
comparative and functional genomics
Language(s) - English
Resource type - Journals
eISSN - 1532-6268
pISSN - 1531-6912
DOI - 10.1002/cfg.382
Subject(s) - mutagenesis , mutant , genome , genetics , computational biology , biology , gene
Three mutant mice with pigmentation phenotypes were recovered from a genome‐wide random mouse chemical mutagenesis study. White toes ( Whto ; MGI:1861986), Belly spot and white toes ( Bswt ; MGI:2152776) and Dark footpads 2 ( Dfp2 ; MGI:1861991) were identified following visual inspection of progeny from a male exposed to the point mutagen ethylnitrosourea (ENU). In order to rapidly localize the causative mutations, genome‐wide linkage scans were performed on pooled DNA samples from backcross animals for each mutant line. Whto was mapped to proximal mouse chromosome (Mmu) 7 between Cen (the centromere) and D7Mit112 (8.0 cM from the centromere), Bswt was mapped to centric Mmul between D1Mit214 (32.1 cM) and D1Mit480 (32.8 cM) and Dfp2 was mapped to proximal Mmu4 between Cen and D4Mit18 (5.2 cM). Whto, Bswt and Dfp2 may provide novel starting points in furthering the elucidation of genetic and biochemical pathways relevant to pigmentation and associated biological processes. Copyright © 2004 John Wiley & Sons, Ltd.