Open AccessGlobal analysis of cell type‐specific gene expression
Author(s) -
Galbraith David W.
Publication year - 2003
Publication title -
comparative and functional genomics
Language(s) - English
Resource type - Journals
eISSN - 1532-6268
pISSN - 1531-6912
DOI - 10.1002/cfg.281
Subject(s) - multicellular organism , cell sorting , cell type , gene expression , computational biology , gene , biology , expression (computer science) , sorting , transgene , flow cytometry , cell , regulation of gene expression , genetics , computer science , algorithm , programming language
The tissues and organs of multicellular eukaryotes are frequently observed to comprise complex three‐dimensional interspersions of different cell types. It is a reasonable assumption that different global patterns of gene expression are found within these different cell types. This review outlines general experimental strategies designed to characterize these global gene expression patterns, based on a combination of methods of transgenic fluorescent protein (FP) expression and targeting, of flow cytometry and sorting and of high‐throughput gene expression analysis. Copyright © 2003 John Wiley & Sons, Ltd.