Global analysis of cell type‐specific gene expression | Zendy

Galbraith David W. | Zendy

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Global analysis of cell type‐specific gene expression

Author(s) -

Galbraith David W.

Publication year - 2003

Publication title -

comparative and functional genomics

Language(s) - English

Resource type - Journals

eISSN - 1532-6268

pISSN - 1531-6912

DOI - 10.1002/cfg.281

Subject(s) - multicellular organism , cell sorting , cell type , computational biology , gene , gene expression , biology , transgene , sorting , expression (computer science) , flow cytometry , cell , genetics , computer science , algorithm , programming language

The tissues and organs of multicellular eukaryotes are frequently observed to comprise complex three‐dimensional interspersions of different cell types. It is a reasonable assumption that different global patterns of gene expression are found within these different cell types. This review outlines general experimental strategies designed to characterize these global gene expression patterns, based on a combination of methods of transgenic fluorescent protein (FP) expression and targeting, of flow cytometry and sorting and of high‐throughput gene expression analysis. Copyright © 2003 John Wiley & Sons, Ltd.

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