Open AccessHepatocyte morphology and kinetics after portal vein embolization
Author(s) -
Komori K.,
Nagino M.,
Nimura Y.
Publication year - 2006
Publication title -
british journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.202
H-Index - 201
eISSN - 1365-2168
pISSN - 0007-1323
DOI - 10.1002/bjs.5332
Subject(s) - medicine , hepatocyte , pathology , liver lobe , hepatectomy , muscle hypertrophy , immunohistochemistry , biology , surgery , resection , in vitro , biochemistry
Background: Macroscopic volume changes after portal vein embolization (PVE) can be assessed accurately by computed tomography, but histological changes remain poorly understood. The aim of this study was to clarify hepatocyte morphology and kinetics after PVE. Methods: The resected livers from 25 patients who underwent extended hepatectomy after PVE and five normal livers were examined using hepatocyte paraffin 1 staining for histomorphometric analysis of hepatocytes. Cell kinetics were determined by Ki‐67 staining and terminal deoxyribonucleotidyl transferase‐mediated dUTP‐digoxigenin nick‐end labelling assay. Kupffer cells were examined by CD68 immunostaining. Results: The number of hepatocytes was similar in the embolized lobe, non‐embolized lobe and normal liver, but hepatocyte volume was greater in the non‐embolized lobe than in the embolized lobe ( P = 0·017). The Ki‐67 labelling index was higher in the non‐embolized lobe ( P < 0·001) whereas the apoptotic index was higher in the embolized lobe ( P < 0·001). There were more Kupffer cells per unit area in the embolized lobe ( P < 0·001). Conclusion: Hepatocyte hypertrophy and replication leads to volume enlargement of the non‐embolized hepatic lobe, whereas hepatocyte atrophy and apoptosis causes a decrease in volume of the embolized lobe. Copyright © 2006 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.