Photophysical processes exploited in digital imaging microscopy: Fluorescence resonance energy transfer and delayed luminescence | Zendy

Jovin T. M. | Zendy; Marriott G. | Zendy; Clegg R. M. | Zendy; ArndtJovin D. J. | Zendy

Open Access

Photophysical processes exploited in digital imaging microscopy: Fluorescence resonance energy transfer and delayed luminescence

Author(s) -

Jovin T. M.,

Marriott G.,

Clegg R. M.,

ArndtJovin D. J.

Publication year - 1989

Publication title -

berichte der bunsengesellschaft für physikalische chemie

Language(s) - English

Resource type - Journals

ISSN - 0005-9021

DOI - 10.1002/bbpc.19890930333

Subject(s) - förster resonance energy transfer , luminescence , phosphorescence , microscopy , fluorescence , fluorescence microscope , materials science , fluorescence lifetime imaging microscopy , microscope , persistent luminescence , resonance fluorescence , energy transfer , confocal , optics , excitation , confocal microscopy , resonance (particle physics) , laser , optoelectronics , chemistry , molecular physics , physics , atomic physics , thermoluminescence , quantum mechanics

The spectroscopic techniques of fluorescence resonance energy transfer and of time‐resolved delayed fluorescence and phosphorescence have been introduced into a microscope equipped with a solid‐state CCD camera and phase‐locked excitation and emission choppers. The distribution and replication of DNA in cells has been quantitated by these methods, as well as by confocal laser scanning microscopy. Sites of replication in living cells can be uniquely identified by delayed luminescence.

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