Open AccessFeasibility of a tetracycline‐binding method for detecting synovial fluid basic calcium phosphate crystals
Author(s) -
Rosenthal Ann K.,
Fahey Mark,
Gohr Claudia,
Burner Todd,
Ko Irina,
Daft Laureen,
Mattson Eric,
Hirschmugl Carol,
Ryan Lawrence M.,
Simkin Peter
Publication year - 2008
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.23882
Subject(s) - oxytetracycline , alizarin red , synovial fluid , staining , chemistry , calcium pyrophosphate , stain , calcium , tetracycline , chromatography , pathology , osteoarthritis , biochemistry , medicine , antibiotics , alternative medicine , organic chemistry
Objective Basic calcium phosphate (BCP) crystals are common components of osteoarthritis (OA) synovial fluid. Progress in understanding the role of these bioactive particles in clinical OA has been hampered by difficulties in their identification. Tetracyclines stain calcium phosphate mineral in bone. The aim of this study was to investigate whether tetracycline staining might be an additional or alternative method for identifying BCP crystals in synovial fluid. Methods A drop of oxytetracycline was mixed with a drop of fluid containing synthetic or native BCP, calcium pyrophosphate dihydrate (CPPD), or monosodium urate (MSU) crystals and placed on a microscope slide. Stained and unstained crystals were examined by light microscopy, with and without a portable broad‐spectrum ultraviolet (UV) pen light. A small set of characterized synovial fluid samples were compared by staining with alizarin red S and oxytetracycline. Synthetic BCP crystals in synovial fluid were quantified fluorimetrically using oxytetracycline. Results After oxytetracycline staining, synthetic and native BCP crystals appeared as fluorescent amorphous aggregates under UV light. Oxytetracycline did not stain CPPD or MSU crystals or other particulates. Oxytetracycline staining had fewer false‐positive test results than did alizarin red S staining and could provide estimates of the quantities of synthetic BCP crystals in synovial fluid. Conclusion With further validation, oxytetracycline staining may prove to be a useful adjunct or alternative to currently available methods for identifying BCP crystals in synovial fluid.