Microautoradiographic study of the intracellular and extracellular distribution of glycerol‐1,3‐C 14 labeled lipids in experimental connective tissue

out on tissue aliquots removed from the same incubation flasks which contained the tissues used for the chemical analysis of glycer01-1,3-C~~ incorporation by granuloma slices 7-450 days of Using the technic of quantitative microautoradiography, it has been possible to identify incorporation of this isotope and study its localization within the several cell types present in polyvinyl sponge and open wound granulomas. In addition, the presence of glycer01-1,3-C~~ in extracellular location has been demonstrated. Using standard histologic methods designed to preserve or extract lipids from the tissue, it has been found that 75 per cent of the incorporated isotope is labile to such procedures. These findings suggest that lipids (lipoproteins) occur as constituents of connective tissue "ground substance." METHODS