Open AccessVisualization of a Limonene Synthesis Metabolon Inside Living Bacteria by Hyperspectral SRS Microscopy
Author(s) -
Zhang Jing,
Shin Jonghyeon,
Tague Nathan,
Lin Haonan,
Zhang Meng,
Ge Xiaowei,
Wong Wilson,
Dunlop Mary J.,
Cheng JiXin
Publication year - 2022
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202203887
Subject(s) - hyperspectral imaging , limonene , synthetic biology , biosynthesis , bacteria , chemistry , colocalization , biophysics , computational biology , biochemistry , microbiology and biotechnology , biology , computer science , enzyme , food science , genetics , artificial intelligence , essential oil
Monitoring biosynthesis activity at single‐cell level is key to metabolic engineering but is still difficult to achieve in a label‐free manner. Using hyperspectral stimulated Raman scattering imaging in the 670–900 cm −1 region, localized limonene synthesis are visualized inside engineered Escherichia coli . The colocalization of limonene and GFP‐fused limonene synthase is confirmed by co‐registered stimulated Raman scattering and two‐photon fluorescence images. The finding suggests a limonene synthesis metabolon with a polar distribution inside the cells. This finding expands the knowledge of de novo limonene biosynthesis in engineered bacteria and highlights the potential of SRS chemical imaging in metabolic engineering research.