Open AccessPhotocatalytic Quantum Dot‐Armed Bacteriophage for Combating Drug‐Resistant Bacterial Infection
Author(s) -
Wang Lei,
Fan Xin,
Gonzalez Moreno Mercedes,
Tkhilaishvili Tamta,
Du Weijie,
Zhang Xianlong,
Nie Chuanxiong,
Trampuz Andrej,
Haag Rainer
Publication year - 2022
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202105668
Subject(s) - pseudomonas aeruginosa , bacteriophage , biofilm , microbiology and biotechnology , infectivity , bacteria , chemistry , multiple drug resistance , antibacterial activity , biology , virology , drug resistance , gene , escherichia coli , virus , biochemistry , genetics
Multidrug‐resistant (MDR) bacterial infection is one of the greatest challenges to public health, a crisis demanding the next generation of highly effective antibacterial agents to specifically target MDR bacteria. Herein, a novel photocatalytic quantum dot (QD)‐armed bacteriophage (QD@Phage) is reported for combating green fluorescent protein‐expressing Pseudomonas aeruginosa (GFP‐ P. aeruginosa ) infection. The proposed QD@Phage nanosystem not only specifically binds to the host GFP‐ P. aeruginosa while preserving the infectivity of the phage itself, but also shows a superior capacity for synergistic bacterial killing by phage and by the photocatalytic localized reactive oxygen species (ROS) generated from anchored QD components. Notably, this highly targeted QD@Phage nanosystem achieves robust in vitro antibacterial elimination for both planktonic (over 99.9%) and biofilm (over 99%) modes of growth. In a mouse wound infection model, this system also shows remarkable activity in eliminating the wound infection and promoting its recovery. These results demonstrate that the novel QD@Phage nanosystem can diversify the existing pool of antibacterial agents and inspire the development of promising therapeutic strategies against MDR bacterial infection.