Open AccessStructures of Class I and Class II Transcription Complexes Reveal the Molecular Basis of RamA‐Dependent Transcription Activation
Author(s) -
Hao Min,
Ye Fuzhou,
Jovanovic Milija,
KottaLoizou Ioly,
Xu Qingqing,
Qin Xiaohua,
Buck Martin,
Zhang Xiaodong,
Wang Minggui
Publication year - 2022
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202103669
Subject(s) - promoter , activator (genetics) , rna polymerase , transcription (linguistics) , gene , biology , bacterial transcription , transcription factor , rna polymerase ii , dna , microbiology and biotechnology , genetics , rna , gene expression , linguistics , philosophy
Transcription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae through controlling genes that encode efflux pumps ( acrA ) and porin‐regulating antisense RNA ( micF ). In bacteria, σ 70 , together with activators, controls the majority of genes by recruiting RNA polymerase (RNAP) to the promoter regions. RNAP and σ 70 form a holoenzyme that recognizes ‐35 and ‐10 promoter DNA consensus sites. Many activators bind upstream from the holoenzyme and can be broadly divided into two classes. RamA acts as a class I activator on acrA and class II activator on micF , respectively. The authors present biochemical and structural data on RamA in complex with RNAP‐ σ 70 at the two promoters and the data reveal the molecular basis for how RamA assembles and interacts with core RNAP and activates transcription that contributes to antibiotic resistance. Further, comparing with CAP/TAP complexes reveals common and activator‐specific features in activator binding and uncovers distinct roles of the two C‐terminal domains of RNAP α subunit.