Open AccessEvaluation of Microfluidic Ceiling Designs for the Capture of Circulating Tumor Cells on a Microarray Platform
Author(s) -
Liu HuiYu,
Koch Claudia,
Haller Anna,
Joosse Simon A.,
Kumar Ravi,
Vellekoop Michael J.,
Horst Ludwig J.,
Keller Laura,
Babayan Anna,
Failla Antonio Virgilio,
Jensen Jana,
Peine Sven,
Keplinger Franz,
Fuchs Harald,
Pantel Klaus,
Hirtz Michael
Publication year - 2020
Publication title -
advanced biosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.153
H-Index - 18
ISSN - 2366-7478
DOI - 10.1002/adbi.201900162
Subject(s) - circulating tumor cell , microfluidics , computer science , ceiling (cloud) , tumor cells , computational biology , nanotechnology , materials science , biology , cancer research , cancer , engineering , genetics , structural engineering , metastasis
The capture of circulating tumor cells (CTCs) is still a challenging application for microfluidic chips, as these cells are rare and hidden in a huge background of blood cells. Here, different microfluidic ceiling designs in regard to their capture efficiency for CTCs in model experiments and more realistic conditions of blood samples spiked with a clinically relevant amount of tumor cells are evaluated. An optimized design for the capture platform that allows highly efficient recovery of CTCs from size‐based pre‐enriched samples under realistic conditions is obtained. Furthermore, the viability of captured tumor cells as well as single cell recovery for downstream genomic analysis is demonstrated. Additionally, the authors' findings underline the importance of evaluating rational design rules for microfluidic devices based on theoretical models by application‐specific experiments.