Open AccessSupporting Survival of Transplanted Stem‐Cell‐Derived Insulin‐Producing Cells in an Encapsulation Device Augmented with Controlled Release of Amino Acids
Author(s) -
Chendke Gauree S.,
Faleo Gaetano,
Juang Charity,
Parent Audrey V.,
Bernards Daniel A.,
Hebrok Matthias,
Tang Qizhi,
Desai Tejal A.
Publication year - 2019
Publication title -
advanced biosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.153
H-Index - 18
ISSN - 2366-7478
DOI - 10.1002/adbi.201900086
Subject(s) - transplantation , insulin , immune system , glutamine , cell encapsulation , islet , stem cell , biology , cell , in vitro , microbiology and biotechnology , immunology , medicine , amino acid , endocrinology , biochemistry
Pancreatic islet transplantation is a promising treatment for type I diabetes, which is a chronic autoimmune disease in which the host immune cells attack insulin‐producing beta cells. The impact of this therapy is limited due to tissue availability and dependence on immunosuppressive drugs that prevent immune rejection of the transplanted cells. These issues can be solved by encapsulating stem cell‐derived insulin‐producing cells in an immunoprotective device. However, encapsulation exacerbates ischemia, and the lack of vasculature at the implantation site post‐transplantation worsens graft survival. Here, an encapsulation device that supplements nutrients to the cells is developed to improve the survival of encapsulated stem cell‐derived insulin‐producing cells in the poorly vascularized subcutaneous space. An internal compartment in the device is fabricated to provide zero‐order release of alanine and glutamine for several weeks. The amino acid reservoir sustains viability of insulin‐producing cells in nutrient limiting conditions in vitro. Moreover, the reservoir also increases cell survival by 30% after transplanting the graft in the subcutaneous space.