Open AccessPoliovirus: Generation and Characterization of Mutants
Author(s) -
Burrill Cecily P.,
Strings Vanessa R.,
Schulte Michael B.,
Andino Raul
Publication year - 2013
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/9780471729259.mc15h02s29
Subject(s) - biology , poliovirus , picornavirus , virology , genome , rna , virus , rna virus , mutant , gene , genetics , complementary dna
Poliovirus (PV) is the prototypical picornavirus. It is a non‐enveloped RNA virus with a small (∼7.5‐kb) genome of positive polarity. cDNA clones of several strains are available, and infectious virus can be produced by the transfection of in vitro–transcribed viral genomes into an appropriate host cell. The ease of genetic studies in poliovirus is a primary reason that it has long served as a model to study RNA virus biology, pathogenesis, and evolution. Protocols for the generation and characterization of PV mutants are presented. Curr. Protoc. Microbiol . 29:15H.2.1‐15H.2.32. © 2013 by John Wiley & Sons, Inc.