Open AccessPoliovirus: Generation, Quantification, Propagation, Purification, and Storage
Author(s) -
Burrill Cecily P.,
Strings Vanessa R.,
Andino Raul
Publication year - 2013
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/9780471729259.mc15h01s29
Subject(s) - biology , poliovirus , virology , rna , picornavirus , virus , genome , hela , titer , complementary dna , transfection , gene , cell culture , genetics
Poliovirus (PV) is the prototypical picornavirus. It is a non‐enveloped RNA virus with a small (∼7.5‐kb) genome of positive polarity. It has long served as a model to study RNA virus biology, pathogenesis, and evolution. cDNA clones of several strains are available, and infectious virus can be produced by the transfection of in vitro transcribed viral genomes into an appropriate host cell. PV infects many human and non‐human primate cell lines including HeLa and HeLa S3 cells, and can grow to high titer in culture. Protocols for the production, propagation, quantification, and purification of PV are presented. Curr. Protoc. Microbiol . 29:15H.1.1‐15H.1.27. © 2013 by John Wiley & Sons, Inc.