Open AccessPreparation of Cell Cultures and Vaccinia Virus Stocks
Author(s) -
Cotter Catherine A.,
Earl Patricia L.,
Wyatt Linda S.,
Moss Bernard
Publication year - 2015
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/9780471729259.mc14a03s39
Subject(s) - vaccinia , trypsinization , virus , biology , virology , poxviridae , orthopoxvirus , cell culture , embryo , dna , microbiology and biotechnology , trypsin , recombinant dna , genetics , gene , biochemistry , enzyme
The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range‐restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2015 by John Wiley & Sons, Inc.