Open AccessListeria monocytogenes : Cultivation and Laboratory Maintenance
Author(s) -
Jones Grant S.,
D'Orazio Sarah E.F.
Publication year - 2013
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/9780471729259.mc09b02s31
Subject(s) - listeria monocytogenes , biology , microbiology and biotechnology , facultative , serotype , listeria , bacteria , genetics , ecology
This unit describes general procedures for the lab cultivation and storage of the Gram‐positive facultative intracellular bacterium Listeria monocytogenes . The basic protocols are relevant for a wide scope of applications including microbial genetics and both in vitro and in vivo infection studies. Commonly used L. monocytogenes strains, serotypes, and growth parameters are also discussed. Curr. Protoc. Microbiol . 31:9B.2.1‐9B.2.7. ©2013 by John Wiley & Sons, Inc.