Open AccessGenetic Manipulation of Campylobacter jejuni
Author(s) -
Davis Lindsay,
Young Kathryn,
DiRita Victor
Publication year - 2008
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/9780471729259.mc08a02s10
Subject(s) - biology , campylobacter jejuni , plasmid , transformation (genetics) , electroporation , isolation (microbiology) , bacterial conjugation , gene , genetics , computational biology , dna , microbiology and biotechnology , bacteria
Molecular manipulation has been a limiting factor in C. jejuni research for many years. Recent advances in molecular techniques adapted for C. jejuni have furthered our understanding of the organism. This unit is dedicated to common molecular tools in bacterial research specifically tailored for C. jejuni . These include colony PCR, DNA isolation, and RNA isolation. The unit also reviews techniques for genetic manipulation, such as the use of plasmids, natural transformation, electroporation, conjugation, and transposition. In addition, a reporter system, the arylsulfatase assay, can be used to study gene expression.