Open AccessGenetic Screens and Biochemical Assays to Characterize Vibrio cholerae O1 Biotypes: Classical and El Tor
Author(s) -
Son Mike S.,
Taylor Ronald K.
Publication year - 2011
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/9780471729259.mc06a02s22
Subject(s) - vibrio cholerae , el tor , biology , cholera , vibrio , microbiology and biotechnology , vibrionaceae , phenotype , pandemic , virology , genetics , bacteria , covid-19 , gene , infectious disease (medical specialty) , medicine , disease , pathology
Vibrio cholerae serogroup O1 has two biotypes, classical and El Tor, the latter of which has displaced the prior and has been the causative agent for the ongoing seventh pandemic. However, reports since 2001 have identified clinical isolates of El Tor that have classical O1 biotype genetic and phenotypic characteristics. These El Tor variants have been emerging in clinical settings with increased frequency, including the 2010 cholera outbreak in Haiti. The emergence of El Tor variants warrants the proper and timely identification of clinical (or environmental) isolates' biotype. This unit describes some quick and simple genetic screens and phenotypic assays (biochemical characterization), to be performed simultaneously, commonly used to distinguish biotype and initiate characterization of any clinical (or environmental) isolates of Vibrio cholerae O1. Curr. Protoc. Microbiol . 22:6A.2.1‐6A.2.17. © 2011 by John Wiley & Sons, Inc.