Open AccessAnalysis of Mouse Growth Plate Development
Author(s) -
Mangiavini Laura,
Merceron Christophe,
Schipani Ernestina
Publication year - 2016
Publication title -
current protocols in mouse biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.77
H-Index - 15
ISSN - 2161-2617
DOI - 10.1002/9780470942390.mo150094
Subject(s) - intramembranous ossification , endochondral ossification , mesenchymal stem cell , cartilage , microbiology and biotechnology , chondrogenesis , skeleton (computer programming) , biology , anatomy , embryogenesis , embryonic stem cell , embryo , genetics , gene
To investigate skeletal development, pathophysiological mechanisms of cartilage and bone disease, and eventually assess innovative treatments, the mouse is a very important resource. During embryonic development, mesenchymal condensations are formed, and cells within these mesenchymal condensations either directly differentiate into osteoblasts and give origin to intramembranous bone, or differentiate into chondrocytes and form a cartilaginous anlage. The cartilaginous anlage or fetal growth plate is then replaced with bone. This process is also called endochondral bone development, and it is responsible for the generation of most of our skeleton. Here we discuss in detail the most common in vivo and in vitro techniques our laboratory is currently using for the analysis of the mouse fetal growth plate during development. © 2016 by John Wiley & Sons, Inc.