Open AccessHigh‐Throughput Screening for Novel Anti‐Infectives Using a C. elegans Pathogenesis Model
Author(s) -
Conery Annie L.,
LarkinsFord Jonah,
Ausubel Frederick M.,
Kirienko Natalia V.
Publication year - 2014
Publication title -
current protocols in chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 14
ISSN - 2160-4762
DOI - 10.1002/9780470559277.ch130160
Subject(s) - caenorhabditis elegans , pathogenesis , biology , pseudomonas aeruginosa , computational biology , microbiology and biotechnology , immunology , gene , genetics , bacteria
In recent history, the nematode Caenorhabditis elegans has provided a compelling platform for the discovery of novel antimicrobial drugs. In this protocol, we present an automated, high‐throughput C. elegans pathogenesis assay, which can be used to screen for anti‐infective compounds that prevent nematodes from dying due to Pseudomonas aeruginosa . New antibiotics identified from such screens would be promising candidates for treatment of human infections, and also can be used as probe compounds to identify novel targets in microbial pathogenesis or host immunity. Curr. Protoc. Chem. Biol . 6:25‐37. © 2014 by John Wiley & Sons, Inc.