Open AccessLuciferase Reporter Assay in Drosophila and Mammalian Tissue Culture Cells
Author(s) -
Yun Chi,
DasGupta Ramanuj
Publication year - 2014
Publication title -
current protocols in chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 14
ISSN - 2160-4762
DOI - 10.1002/9780470559277.ch130149
Subject(s) - luciferase , rna interference , functional genomics , gene silencing , biology , crispr , computational biology , reporter gene , gene , function (biology) , microbiology and biotechnology , genome , gene expression , genomics , genetics , transfection , rna
Luciferase reporter gene assays are one of the most common methods for monitoring gene activity. Because of their sensitivity, dynamic range, and lack of endogenous activity, luciferase assays have been particularly useful for functional genomics in cell‐based assays, such as RNAi screening. This unit describes delivery of two luciferase reporters with other nucleic acids (siRNA/dsRNA), measurement of the dual luciferase activities, and analysis of data generated. The systematic query of gene function (RNAi) combined with the advances in luminescent technology have made it possible to design powerful whole genome screens to address diverse and significant biological questions. Curr. Protoc. Mol. Biol . 6:7‐23. © 2014 by John Wiley & Sons, Inc.