Open AccessAdvances in Lectin Microarray Technology: Optimized Protocols for Piezoelectric Print Conditions
Author(s) -
Pilobello Kanoelani T.,
Agrawal Praveen,
Rouse Richard,
Mahal Lara K.
Publication year - 2013
Publication title -
current protocols in chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 14
ISSN - 2160-4762
DOI - 10.1002/9780470559277.ch120035
Subject(s) - lectin , glycan , glycobiology , glycomics , dna microarray , microarray , fucose , sialic acid , glycosylation , gene chip analysis , biology , epitope , antibody microarray , computational biology , biochemistry , microbiology and biotechnology , chemistry , galactose , antibody , glycoprotein , gene , gene expression , immunology
Lectin microarray technology has been used to profile the glycosylation of a multitude of biological and clinical samples, leading to new clinical biomarkers and advances in glycobiology. Lectin microarrays, which include >90 plant lectins, recombinant lectins, and selected antibodies, are used to profile N ‐linked, O ‐linked, and glycolipid glycans. The specificity and depth of glycan profiling depends upon the carbohydrate‐binding proteins arrayed. The current set targets mammalian carbohydrates including fucose, high mannose, branched and complex N ‐linked, α‐ and β‐galactose and GalNAc, α‐2,3‐ and α‐2,6‐sialic acid, LacNAc, and Lewis X epitopes. Previous protocols have described the use of a contact microarray printer for lectin microarray production. Here, an updated protocol that uses a non‐contact, piezoelectric printer, which leads to increased lectin activity on the array, is presented. Optimization of print and sample hybridization conditions and methods of analysis are discussed. Curr. Protoc. Chem. Biol . 5:1‐23 © 2013 by John Wiley & Sons, Inc.