Open AccessLabeling Substrate Proteins of Poly(ADP‐ribose) Polymerases with Clickable NAD Analog
Author(s) -
Jiang Hong,
Lin Hening
Publication year - 2012
Publication title -
current protocols in chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 14
ISSN - 2160-4762
DOI - 10.1002/9780470559277.ch110196
Subject(s) - nad+ kinase , poly adp ribose polymerase , nicotinamide adenine dinucleotide , polymerase , biochemistry , dna , dna repair , enzyme , adp ribosylation , chemistry , rna , biology , computational biology , microbiology and biotechnology , gene
Poly(ADP‐ribose) polymerases (PARPs) play important roles in various biological processes, including DNA repair, transcriptional regulation, mitosis, and RNA processing. PARP inhibitors are in clinical trials for treating human cancers. Understanding the biological function of PARPs will be important to fully realize the therapeutic potential of PARP inhibitors. We have developed a clickable analog of nicotinamide adenine dinucleotide (NAD) that can be used for in‐gel visualization, affinity purification, and identification of substrate proteins of PARPs. The protocols in this article describe a general procedure to label substrate proteins of PARPs using the clickable NAD analog. Curr. Protoc. Chem. Biol . 4:19‐34 © 2012 by John Wiley & Sons, Inc.