Open AccessDerivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applications
Author(s) -
Song Xuezheng,
HeimburgMolinaro Jamie,
Smith David F.,
Cummings Richard D.
Publication year - 2011
Publication title -
current protocols in chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 14
ISSN - 2160-4762
DOI - 10.1002/9780470559277.ch100194
Subject(s) - glycan , derivatization , glycomics , chemistry , biochemistry , microarray , computational biology , chromatography , mass spectrometry , biology , glycoprotein , gene , gene expression
Nature possesses an unlimited number and source of biologically relevant natural glycans, many of which are too complicated to synthesize in the laboratory. To capitalize on the naturally occurring plethora of glycans, a method is presented here to fluorescently tag isolated free glycans while maintaining the closed‐ring structure. After purification of the labeled glycans, they can be printed on a glass surface to create a natural glycan microarray, suitable for interrogation with potential glycan‐binding proteins. The derivatization of these natural glycans has vastly expanded the number of glycans available for functional studies. Curr. Protoc. Chem. Biol . 3:53‐63 © 2011 by John Wiley & Sons, Inc.