Open AccessActivity‐Based Protein Profiling (ABPP) and Click Chemistry (CC)–ABPP by MudPIT Mass Spectrometry
Author(s) -
Speers Anna E.,
Cravatt Benjamin F.
Publication year - 2009
Publication title -
current protocols in chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 14
ISSN - 2160-4762
DOI - 10.1002/9780470559277.ch090138
Subject(s) - click chemistry , proteome , profiling (computer programming) , chemistry , mass spectrometry , computational biology , proteomics , biochemistry , chromatography , combinatorial chemistry , computer science , biology , gene , operating system
Activity‐based protein profiling (ABPP) is a chemical proteomic method for functional interrogation of enzymes within complex proteomes. This unit presents a protocol for in vitro and in vivo labeling using ABPP and Click Chemistry (CC)‐ABPP probes for in‐depth profiling using the Multi‐dimensional Protein Identification Technology (MudPIT) analysis platform. Curr. Protoc. Chem Biol . 1:29‐41. © 2009 by John Wiley & Sons, Inc.