Open AccessDifferentiation of Hepatocytes from Pluripotent Stem Cells
Author(s) -
Mallanna Sunil K.,
Duncan Stephen A.
Publication year - 2013
Publication title -
current protocols in stem cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 28
eISSN - 1938-8969
pISSN - 1941-7322
DOI - 10.1002/9780470151808.sc01g04s26
Subject(s) - induced pluripotent stem cell , embryonic stem cell , biology , stem cell , microbiology and biotechnology , hepatocyte , cellular differentiation , kosr , drug discovery , directed differentiation , bioinformatics , genetics , in vitro , gene
Differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells into hepatocyte‐like cells provides a platform to study the molecular basis of human hepatocyte differentiation, to develop cell culture models of liver disease, and to potentially provide hepatocytes for treatment of end‐stage liver disease. Additionally, hepatocyte‐like cells generated from human pluripotent stem cells could serve as platforms for drug discovery, determination of pharmaceutical‐induced hepatotoxicity, and evaluation of idiosyncratic drug‐drug interactions. Here, we describe a step‐wise protocol previously developed in our laboratory that facilitates the highly efficient and reproducible differentiation of human pluripotent stem cells into hepatocyte‐like cells. Our protocol uses defined culture conditions and closely recapitulates key developmental events that are found to occur during hepatogenesis. Curr. Protoc. Stem Cell Biol . 26:1G.4.1‐1G.4.13. © 2013 by John Wiley & Sons, Inc.