Open AccessIsoflurane induces Art2‐Rsp5‐dependent endocytosis of Bap2 in yeast
Author(s) -
Kozu Fumi,
ShirahamaNoda Kanae,
Araki Yasuhiro,
Kira Shintaro,
Niwa Hitoshi,
Noda Takeshi
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13302
Subject(s) - isoflurane , endocytosis , ubiquitin ligase , ubiquitin , microbiology and biotechnology , yeast , chemistry , anesthetic , pharmacology , biochemistry , biology , anesthesia , medicine , receptor , gene , organic chemistry
Although general anesthesia is indispensable during modern surgical procedures, the mechanism by which inhalation anesthetics act on the synaptic membrane at the molecular and cellular level is largely unknown. In this study, we used yeast cells to examine the effect of isoflurane, an inhalation anesthetic, on membrane proteins. Bap2, an amino acid transporter localized on the plasma membrane, was endocytosed when yeast cells were treated with isoflurane. Depletion of RSP5 , an E3 ligase, prevented this endocytosis and Bap2 was ubiquitinated in response to isoflurane, indicating an ubiquitin‐dependent process. Screening all the Rsp5 binding adaptors showed that Art2 plays a central role in this process. These results suggest that isoflurane affects Bap2 via an Art2‐Rsp5‐dependent ubiquitination system.